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Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.
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Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.
Dried Gel Strips With An Immobilized Ph Gradient Serva Ipg Bluestrips, 3 10/11, supplied by SERVA Electrophoresis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gradient gel strips  (Cytiva Europe)
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Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.
Gradient Gel Strips, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dried gel strips with an immobilized ph gradient serva ipg bluestrips, 3-10 /  (SERVA Electrophoresis)
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SERVA Electrophoresis dried gel strips with an immobilized ph gradient serva ipg bluestrips, 3-10 /
Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.
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Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.
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immobilized ph gradient (ipg) gel strips ph 3-10  (Bio-Rad)
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Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.
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immobilized ph gradient gel strips immobiline drystrip, ph 3 to 10, nonlinear  (Amersham Pharmacia Biotech Ltd)
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Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.
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ph 3–10 nonlinear immobilized ph gradient (ipg) gel strip  (Bio-Rad)
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Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.
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ph 3–10 nonlinear immobilized ph gradient (ipg) gel strip - by Bioz Stars, 2026-08
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ph gradient gel ipg strips  (Cytiva Europe)
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Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.
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24-cm immobilised ipg gel strip linear ph gradient 3–10  (Agilent technologies)
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Agilent technologies 24-cm immobilised ipg gel strip linear ph gradient 3–10
Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.
24 Cm Immobilised Ipg Gel Strip Linear Ph Gradient 3–10, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.

Journal: PLoS ONE

Article Title: Proteomic Changes during B Cell Maturation: 2D-DIGE Approach

doi: 10.1371/journal.pone.0077894

Figure Lengend Snippet: Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.

Article Snippet: The IEF was carried out using immobilized nonlinear pH gradient 3–10 gel strips (24 cm Immobiline DryStrip gels, GE Healthcare) and Ettan II IPGphor system (GE Healthcare) for a total of 40–50 kVh.

Techniques: Expressing